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Optimization of cellulase production using

Saravanan P, Muthuvelayudham R, Rajesh Kannan R, Viruthagiri T

《化学科学与工程前沿（英文）》 2012年第6卷第4期页码 443-452 doi: 10.1007/s11705-012-1225-1

摘要： The potential of for cellulase production using pineapple waste as substrate has been investigated. A maximum cellulase activity of 9.23 U/mL is obtained under the optimum experimental conditions: pH (5.5), temperature (37.5°C), initial substrate concentration (3%), inoculum concentration (6.6 × 10 ?CFU/mL), and culture time (6 days). Box-Behnken design (BBD) statistical tool and genetic algorithm (GA) are used to optimize the process parameters. The BBD study of linear and quadratic interactive effects of experimental variables on the desired response of cellulase activity showed that the second order polynomial is significant ( = 0.9414). The experimental cellulase activity under the optimal conditions identified by the BBD is 9.23 U/mL and that by GA is 6.98 U/mL. This result indicates that the BBD model gives better result than GA in the present case.

关键词： cellulase pineapple waste Trichoderma reesei Box-Behnken design genetic algorithm

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Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

《农业科学与工程前沿（英文）》 2018年第5卷第2期页码 271-279 doi: 10.15302/J-FASE-2018214

摘要： This study reports a strain of CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, , and with a pivotal role in tomato ( ) plant growth enhancement. A total of 254 isolates were screened by antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of . The antagonistic assay and greenhouse experiments indicated that CCTCC-SBW0162 gave the highest inhibition rate (90.6%) and disease reduction (80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against , molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of . The molecular docking results indicated that the combination of harzianopyridone, harzianolide and anthraquinone C derived from CCTCC-SBW0162 could synergistically improve antifungal activity against through the inhibition/modification of pathogenicity and virulence related proteins. However, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect derived metabolites against the proteins of and their interactions.

关键词： anthraquinone Botrytis cinerea harzianolide harzianopyridone molecular docking Trichoderma harzianum